SalmonellaDetection in Meat and Fish by Membrane Hybridization with Chromogenic/Phosphatase/Biotin DNA Probe

摘要

ABSTRACTThe hybridization specificity of a biotin labeled 1.8 kb HindIII DNA fragment was confirmed by colony hybridization withSalmonellaandnon‐Salmonellaisolates. Culture conditions were then tested for the enrichment of salmonellae in foods with large populations of competitive flora. Different conditions of preenrichment and selective enrichment could be used for detecting low populations of salmonellae in foods. Enrichment using lactose combined tetrathionate (CTET) broth and Salmonella‐Shigella (SS) agar followed by spotting the suspected isolates on a nitrocellulose membrane CETE → SS (S) was better. As low as 1.6 × 100salmonellae/g food could be de