Abstract AdipoRl is one of the adiponectin receptors which are important for adiponectin signaling. Because adiponectin is a candidate gene for common obesity, it is also hypothesized that variations in AdipoRl may be involved in the development of complex obesity. Therefore, we designed an association study for the AdipoRl gene. We performed a case-control association study including 1,021 obese subjects (mean age 42 +- 12 years; mean BMI 38.2 +- 6.2 kg/m2) and 226 lean, healthy individuals (mean age 36 db 7 years; mean BMI 22.1 +-1.7 kg/m2). Nine tagSNPs were selected to cover the entire AdipoRl gene and surrounding 7 kb region (based on HapMap data). TagSNPs were genotyped using Acyclo-Prime-Fluorescence Polarization (FP) SNP Detection kits and TaqMan Pre-Designed SNP Genotyping assays according to manufacturer's protocols. We found that the rslO75399 non-reference allele decreases obesity risk by 45 in men only odds ratio (OR) = 0.55, 95 CI 0.35-0.87, nominal P = 0.010. However, after Bonferroni correction for multiple testing, this association is lost. None of the other tagSNPs were associated with obesity when studying the entire population, nor when looking at men and women separately. Quantitative analysis of the effect of each SNP on height, weight, and BMI revealed that none of the tagSNPs are associated with weight or BMI. We report here that we found no decisive evidence for association between AdipoRl tagSNPs and complex obesity in our Belgian Caucasian population.
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