A Single and Simple Receptor as a Multifunctional Chemosensor for the Al3+/Cu2+ ions and Its Live Cell Imaging Applications

摘要

A simple and smart chemosensor (DSA) has been developed using Schiff base ligand for highly sensitive and selective colorimetric detection of Al3+/Cu2+ in partial aqueous DMSO mixture (1: 1) as a solvent medium. It showed a rapid fluorescence response (similar to 10 Sec) with Al3+ as greenish yellow from weak orange fluorescence while, Cu2+ ions affords yellow colour from fade orange colour and it can be sensed even by naked eyes. Besides showing great selectivity, the probe gives a lower sensitivity of 20 nM for Al3+ and 0.27 mu M for Cu2+ in the fluorescence and UV-vis. spectral studies respectively. The solid state colorimetric and fluorescence changes of the probe were examined in silica supported TLC plates. The sensing mechanism of the proposed probe in the presence of Al3+ and Cu2+ was determined with the support of a job's plot, H-1-NMR, ESI-Mass and theoretical (DFT) analysis. Based on the observed results from the absorbance and fluorescence spectroscopic studies, a logic circuit was designed with the combination of OR, NOT and AND logic gates. Moreover, the probe DSA has been employed for live cell imaging to detect Al3+ ions in C6 glioma cells and it showed a good cell permeability and efficiency in the detection of Al3+ ions.