ABSTRACTAntimiciobials were evaluated in thioglycollate broth at pH 6.5 for the ability to inhibit growth and toxin production byC. botulinum12885A and ATCC 7949 (Type B). Methyl, ethyl, propyl, and butyl parabens (0.1) and sorbic acid (0.2) were effective in inhibiting growth ofC. botulinum12885A and ATCC 7949 in broth. Ethyl, propyl, and butyl parabens (0.1) and sorbic acid (0.2) inhibited toxin production by both strains in culture medium. Ethyl, propyl, butyl parabens (0.1) and sorbic acid (0.2) were individually added to a comminuted pork slurry having salt and sugar, with or without 40 ppm sodium nitrite. Cans were inoculated with a mixture ofC. botulinum12885A and ATCC 7949 spores. The canned product was abused by holding at 27°C and was observed over a 3‐month period for swollen cans. Swollen cans were examined for botulinal toxin by the mouse bioassay. Propyl and butyl paraben did not inhibit or delay toxin production. Ethyl paraben with or without nitrite delayed toxin production for 4 wk. Sorbic acid inhibited toxin for 3 wk; when 40 ppm nitrite was added to the sorbic acid treatment, toxin production was delayed for 4
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