Malic Acid Analysis in Cucumber Juice and Fermentation Brines in the Presence of Interfering Fructose

摘要

ABSTRACTA procedure was developed for HPLC analysis of malic acid in the presence of an interfering fructose peak with an Aminex HPX‐87H column. Fructose in cucumber juice or fermented cucumber brine was reduced to mannitol and sorbitol with sodium borohydride. The sugar alcohols eluted after malic acid and did not absorb light at 210 nm so that malic acid could be quantitatively determined either by refractive index or ultraviolet detectors. Lactic acid, acetic acid, and ethanol could also be determined in the sample after reduction of fructos