Thin‐Layer Chromatographic Separation of Beet Pigments

摘要

ABSTRACTA new simple method for the thin‐layer chromatographic (TLC) separation of betalaines has been developed and is described in this paper, The individual components were separated by preparative TLC on cellulose‐coated plates and analyzed by a multiple development TLC technique with solvent mixtures consisting of ethanol, isoppropanol, water, and acetic acid. Separated components of various hues were clearly visible after development without application of an indicator. Spectral analyses of fractions also were conducted. By this TLC procedure, analytical data were obtained more rapidly and conveniently than could be obtained by other methods such as electrophore