ABSTRACTThe effects of storage time (0, 15, 35, and 56 days), storage temperature (−29°C and 5°C), and storage solution (distilled water, Butterfield's buffer and 0.85 saline) on the resistance ofBacillus subtilisvar.nigerspores to 5 hydrogen peroxide heated at 50°C were evaluated. None of the storage conditions had a significant effect on the resistance of spores exposed to hydrogen peroxide for 5 min or less. When 8 min of exposure to H2O2was used, storage temperature and storage solution had significant effects on spore resistance. Storage time had a significant quadratic effect on resistance; spores stored at –29°C were more resistant than spores stored at 4°C; and spores stored in 0.85 sodium chloride were more resistant than spores stored in distilled water and Butterfield's buffer. There was a significant storage temperature by storage solution interaction after a 10‐min exposure. Variability in spore resistance during storage was less for spores stored at 4°C than for spores stor
展开▼
