Some Solvent Systems for the Resolution of Dansyl Amino Acids by Silicagel Thin Layer Chromatography

摘要

The dansyl amino acids have been divided into two groups depending on their chromatographic behaviour on silicagel thin layers in ten new solvent systems, five for each group. The systems reported herein provide rapid and effective resolution (35#x2013;45 min) for 20 dansyl amino acids in all. The systems also provide separations of Dns-Thr/Dns-Ser, Dns-Asp/Dns-Glu and Dns-Arg/Dns-#x221E; lys which were earlier reported 13 to be unresolved even by two-dimensional thin layer chromatography.