ABSTRACTTWO methods for phosvitin preparation were evaluated, based on purity of the products. Phosvitin was found to be heterogeneous in the polyacrylamide‐gel electropherogram in which it migrated into two components designated α and β‐phosvitin. They were partially fractionated using gel filtration. The β‐phosvitin contained 9.20 phosphorus, while α had 2.97. Phosvitin was dissociated into several bands ranging in molecular weights from 18,500—60,000 daltons, in the SDS polyacrylamide‐gel electropherogram. Results indicate that phosvitin is composed of several subcomponents or polypeptides which interact or polymerize in aqueous solution to form larger molecular weig
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