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Release of Single‐Cell Protein by Induced Cell Lysis

机译:Release of Single‐Cell Protein by Induced Cell Lysis

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ABSTRACTA temperature sensitive lysis mutant ofSaccharomyces cerevisiaeJD 109, which grows normally at 27°C, stops growing and partially lyses at a nonpermissive temperature (37°C). In studies in a 14L fermentor with temperature shifted (from 27°C to 37°C) at 3.0 g/L cells, the effect of pH was investigated with the result that the rate of lysis is faster at pH 8 than at pH 5.6; the protein in the cells decreases from 45 to 35. When both pH (from 5.6 to 8.0) and temperature are shifted, the culture showed a more noticeable inhibition of growth and lysis than when only pH was changed and the temperature was maintained at 2

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