Callus Formation and Plant Regeneration from Protoplasts Derived from Suspension Culture of Rice (Oryzasativa L. cv. ‘Taipei 177’)

摘要

AbstractProtoplasts were isolated from young inflorescence‐derived suspension cultures of a japonica rice cultivar ‘Taipei 177’. The isolated protoplasts which were cultured either in liquid, agar on Sea plaque agarose underwent sustained division. Maximum plating efficiency of 1.06 occurred in a medium containing macroelements of KM, microelements and vitamins of B5, 0.5 Sea plaque agarose, 1.0 mg/l of 2,4‐D, and glucose as an osmotic stabilizer. Green and albino plants were regenerated from the protocalli in MS semisolid medium containing 4 mg/l BAP, 0.5 mg/l NAA and 500 mg/l casein hydrolysate (