ABSTRACTAn enterotoxin A (SEA) producing strain ofStaphylococcus aureuswas inoculated onto mushrooms and incubated under simulated pre‐and post‐canning conditions.S. aureusgrew and produced SEA in mushrooms incubated at 25°C in 10 to 20 NaCl. Growth and SEA production occurred when mushrooms were stored in plastic bags at 37°C, but not at 25°C. Mushrooms heat‐processed at 121.1°C supportedS. aureusgrowth and SEA production. No SEA was recovered from mushrooms inoculated with 1 or 10 ng SEA/g and heated at 121.1°C for 4.5 min. At higher inoculated SEA levels (100 and 1,000 ng/g), SEA was detectable after 10 min of heating. Staphylococcal enterotoxin could be present in canned mushrooms as a result of pre‐, but more likely post‐processing
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