COMPARISON OF Tetrahymena pyriformis W AND RAT BIOASSAYS FOR THE DETERMINATION OF PROTEIN QUALITY

摘要

ABSTRACTThe protein quality of 34 samples of commercially prepared foods was determined using theTetrahymena pyriformisW and rat Protein Efficiency Ratio (PER) bioassays. ANRC Reference Casein was used as the reference protein in both assays. The regression equation relating theTefrahymenaRelative Nutritive Value (RNV) and PER was: PER = 0.286 + 0.022 (RNV), r = 0.90 (P<0.01). This relationship could be used to predict the protein quality of food samples. The use of the Coulter particle counter instead of the direct microscopic counting procedure increased the efficiency of theTetrahymenaassay by reducing counting error and the length of time required. In addition, quantitative information on cell size in relation to quality and quantity of protein in the test sample was obtained. The results of these studies suggest that theTetrahymena pyriformisW assay may be adapted to provide a rapid, low cost assay for estimation of protein quality of commercially prepared foods.