Development and characterization of microsatellite markers for Prunus verecunda and Prunus grayana (Rosaceae)

摘要

Simple sequence repeat (SSR) markers were developed for Prunus verecunda and Prunusgrayana to help understand the seed dispersal pattern of each species. We isolated and characterized nine microsatellite loci (four from P. verecunda and five from P. grayana). In P. verecunda, the number of alleles detected and the expected heterozygosity of five loci ranged from 11 to 24 and 0. 59 to 0. 92, respectively. In P. grayana, the number of alleles detected and the expected heterozygosity of five loci ranged from 4 to 14 and 0. 62 to 0. 86, respectively. These results show that the markers described here will be useful in studying the seed dispersal pattern of Prunus species.