Right coronary artery ligation in mice: a novel method to investigate right ventricular dysfunction and biventricular interaction

摘要

Right ventricular (RV) dysfunction can lead to complications after acute inferior myocardial infarction (MI). However, it is unclear how RV failure after Ml contributes to left-sided dysfunction. The aim of the present study was to investigate the consequences of right coronary artery (RCA) ligation in mice. RCA ligation was performed in C57BL/6JRJ mice (n = 38). The cardiac phenolypes were characterized using high-resolution echocardiography performed up to 4 wk post-RCA ligation. In faret size was measured using 2,3,5-triphenyltetrazolium chloride staining 24 h post-RCA ligation, and the extent of the fibrotic area was determined 4 wk after Ml. RV dysfunction was confirmed 24 h post-RCA ligation by a decrease in the tricuspid annular plane systolic excursion (P < 0.001) and RV longitudinal strain analysis (P < 0.001). Infarct size measured ex vivo represented 45.1 ± 9.1 of the RV free wall. RCA permanent ligation increased the RV-to-left ventricular (LV) area ratio (P < 0.01).