ABSTRACTThe Davis method for estimating hesperidin in orange juice was shown to give results which were highly dependent upon reaction time, wavelength and sample preparation methods. The indicated hesperidin values obtained by the published method (5 min reaction time) were 10 higher than after 10 min with a corresponding standard curve. Hesperidin values were lower when the absorbance was measured at 360 nm (maximum for hesperidin chalcone) than at 420 nm (Davis procedure). All samples treatments to reduce pulp content tended to lower the results as compared with whole juice. The effects were greater when the absorbance was measured at 360 nm than 420 nm.
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