The aim of this study was to evaluate antican- didal activity of H. elengatum L. var. elengatum es- sential oil against ten human pathogenic Candida strains. Aerial parts of Hypericzmz elongatum L. var. elongatzmz was hydrodistilled for 3 11 using a Clevenger-type apparatus to produce essential oil in 02 yield. The oil was subsequently analyzed by gas chrornatography (GC) and gas chromatography- mass spectrometly (GC-MS) systems simultane- ously. Main constituents of the oil were identified as oc-pi11e11e(37.4)_. (E)-3-ocimene (23.6) and 3-pi- nene (10), respectively. The essential oil and its main constituents or-pinene, ocimene were evaluated for their anticandidal activity against by using partly modified CLSI M27-A2 in vitro broth microdilution method. Minimum inhibitory concentrations (MIC) results against tested strains between 31 to 1000 ug/mL. Remarkably, the most susceptible strain was determined as C. tropicalis at 31ug/1uL. H. elongatum var. elongalsum essential oil is a promising natural mixture that is capable of inhibit- ing the growth of C. tropicalis in relative low con- centrations.
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机译：本研究的目的是评估 H. elengatum L. var. elengatum es- sential 油对 10 种人类致病性念珠菌菌株的抗念珠菌活性。使用Clevenger型装置对Hypericzmz elongatum L. var. elongatzmz的地上部分进行加氢蒸馏3 11，以02%的收率生产精油。随后通过气相色谱法（GC）和气相色谱-质谱法（GC-MS）系统同时对油进行分析。该油的主要成分被鉴定为oc-pi11e11e（37.4%）_。（E）-[3-噻吩（23.6%）和[3-庚烯（10%）。使用部分改良的CLSI M27-A2体外肉汤微量稀释法评估精油及其主要成分或蒎烯，ocimene的抗念珠菌活性。针对 31 至 1000 ug/mL 之间测试菌株的最低抑菌浓度 （MIC） 结果。值得注意的是，最易感的菌株被确定为热带梭菌，浓度为31ug/1uL。H. elongatum var. elongalsum 精油是一种很有前途的天然混合物，能够在相对较低的浓度下抑制热带梭菌的生长。
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