Application of Response Surface Methodology (RSM) to theIn VitroMeasurement of Bile Salt Binding Under Physiological Conditions

摘要

ABSTRACTResponse surface methodology was used to assess the effects of adjusting incubation time, sample weight and buffer pH on thein vitromeasurements of sodium glycocholate binding by ground and un‐ground AACC wheat bran. The degree of bile salt binding could be increased by increasing incubation time and by reducing buffer pH. Maximum binding occurred if the sample weight per incubation were approximately 200 mg. Reducing particle size slightly modified the effects of time and pH. Washing the wheat bran fibers with phosphate buffer reduced the total quantity of bile salts bound; however, response surface trends were not affecte