Wide‐Bore Capillary Gas Chromatographic Method for Quantification of Cholesterol Oxidation Products in Egg Yolk Powder

摘要

ABSTRACTA wide‐bore capillary gas chromatographic (GC) method was developed for quantification of cholesterol oxidation products in egg yolk powder. Baseline separations were achieved between 7α‐ and 7β‐hydroxycholesterol, cholesterol, cholesterol‐5α,6α‐epoxide, cholesterol‐5β,6β‐epoxide and 7‐ketocholesterol chromatographed as the trimethylsilylated sterols. Excellent response linearity was obtained for each cholesterol oxidation product, permitting reliable quantification. Recoveries from freeze‐dried egg yolk spiked with various levels of cholesterol oxidation products ranged from 78.2 to 95.1. The coefficients of variation compared favorably to those for p