Extraction of Milk Clotting Enzyme from Sodom Apple(Calotropis procera)

摘要

ABSTRACTA milk clotting enzyme with low proteolytic activity was extracted with ammonium sulfate, at 0.40‐0.65 saturation, from sodom apple leaves. The enzyme with apparently a basic isoelectric point was activated by cysteine and was more active at 65°C than at 35°C. Milk clotting activity increased with pH at 65°C, with the enzyme being almost twice as active at pH 6.4 as at pH 5.4‐5.7. Storage at 4°C for 15 days resulted in a 30‐50 loss in enzyme