The immobilization of enzymes onto hydrolyzed polyethylene‐g‐co‐2‐HEMA

摘要

AbstractA study of the immobilization of trypsin and other enzymes onto hydrolyzed poly(2‐hydroxyethyl methacrylate)‐g‐co‐polyethylene using hydroxyl and carboxyl activating agents has been undertaken. Some emphasis was placed on the immobilized trypsin system which involved examination of the variation of (i) the extent of hydrolysis of the graft copolymer, (ii) the concentration of activating agent, and (iii) the temperature of coupling. With the trypsin system, an increase in carbodi‐imide concentration gave an increase in the amount of protein immobilized but a marked decrease in the retention of enzymic activity. Comparison of the behavior of the free with the immobilized enzyme showed that satisfactory yields were obtained and the immobilized system has an extended pH profile and good stability and thus would have broad applicability. The kinetic factors were examined further, and the role of the graft copolymer chains in the immobilized system is