Isolation and characterization of polymorphic microsatellite markers in the grasshopper Mioscirtus wagneri (Orthoptera: Acrididae)

摘要

Eight polymorphic microsatellite markers were developed for the grasshopper Mioscirtus wagneri. Polymorphism at these loci was evaluated in 25 individuals from Central Spain. The number of alleles per locus ranged from 3 to 9 and their observed and expected heterozygosities ranged from 0. 28 to 0. 80 and 0. 25 to 0. 84, respectively. All genotypic frequencies conformed to Hardy-Weinberg equilibrium expectations, with no evidence of genotypic linkage disequilibrium between any pair of loci. These loci will be highly useful for the study of the population genetic structure and diversity of this grasshopper species forming highly fragmented populations of great conservation concern.