MORPHOLOGICAL CHANGES INDUCED IN HIRSUTELLA GIGANTEA PETCH BY ETHYLENEDIAMINETETRAACETATE

摘要

The addition of ethylenediaminetetraacetic acid (EDTA) at a level of 6ensp;times;ensp;10minus;5ensp;Mto a glucose ndash; yeast extract agar medium inhibits the growth ofHirsutella gigantea. At a concentration of 3ensp;times;ensp;10minus;3ensp;M, the mycelium undergoes a morphological change and yeast-like, single cells are formed. These effects may be attributed to the chelating properties of EDTA since they can be reversed by the addition of the metal ions Mn++, Fe++, or Ca++.