Naive T cells can mediate delayed‐type hypersensitivity response in T cell receptor transgenic mice

摘要

AbstractWe produced transgenic mice expressing Tcell receptor‐αβ chain genes, derived from the chicken ovalbumin (OVA)‐specific I‐Ad‐restricted CD4+CD8−T helper cell clone 7–3–7. In transgenic mice with H‐2dgenetic background (Tg‐d mice), delayed‐type hypersensitivity (DTH) was induced in the hind footpad by one inoculation with OVA without any previous sensitization, suggesting that naive T cells have the potential to be involved in DTH response. Spleen cells from nonimmunized Tg‐d mice showed a strong T cell proliferative response toin vitrostimulation with OVA. Furthermore, these spleen cells produce cytokines including interleukin(IL)‐2, IL‐3, interferon‐γ, granulocyte/macrophage colony‐stimulating factor, macrophage inflammatory protein (MIP)‐1α and MIP‐1β, which may play an important role in the attraction of mononuclea