AbstractPlant regeneration from callus cultures ofAllium trifoliatumsubsp.hirsutumfertile accession F‐370, was studied as a means for clonal multiplication and germplasm storage ofAlliumspp. Callus was induced onin votro‐cultured basal leaf explants. Best proliferation was obtained on modified BDS medium supplemented with (mg/1): 0.75 picloram, 2.0 benzyl adenine, and 900 casein hydrolysate. Shoot and root organogenesis were obtained in 3 to 5 month old subcultured calli, on BDS or MS medium supplemented with (mg/1): either 0.03 picloram or no auxin, 2 BA or 2‐isopentenyladenine, and 900 casein hydrolysate. Direct bulb formation, without shoot elongation, occurred on BDS medium with 10 mg/1 IBA. Under these conditions, callus formation and organogenesis were not obtained withA. trifoliatumsubsp.hirsutumvar. sterile, a male‐sterile genotype. Most regenerants were phenotypically normal, but some abnormal shoots were also observed, i.e. shoots with vitrified or extremely broad leaves. Isozyme polymorphism analysis of seven proteins in the latter regenerants, and in several callus cultures, revealed significant deviation from the original pattern in esterase, 6‐phosphogluconate dehydrogenase and superoxide dismutase. No such deviations were detected in normal regenerat
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