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MULTI-METAL RESISTANCE, As-DETOXIFICATION AND HYDROCARBON DEGRADATION BY BACILLUS SPECIES

机译:抗多金属性、砷解毒和烃类降解

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摘要

Arsenic (As) is a well-known toxic metalloid which has found its way to ground waters in many parts of the world, causing severe health problems. As-resistant bacteria are environment friendly tools to remediate such problems. In this study, As(V) resistant bacteria were isolated from industrial site of Kasur, Pakistan. 16S rDNA sequencing revealed the identification of isolates S1 and S5 as Bacillus sub-tilis and Bacillus cereus, respectively. Isolates B. subtilis S1, S2 and S8 were able to resist 1.0mM Zn, Co, Ni, Pb, Cu and 0.1mM Cr and Hg. Bacterial isolates S2, B. cereus S5, S7 and S8 were able to resist As(V) up to 100mM. Maximum As(V)-reduction was shown by B. cereus S5, up to 837.6^M (83.7). Maximum As(III) oxidation was exhibited by B. subtilis S1, up to 122.44^M (24.4). The bacterial isolates were also found to produce biosurfactants, and maximum emulsification index (up to 37.5) was shown by bacterial isolate B. subtilis S1. Such bacteria are potential candidates for bioremediation purposes of sites contaminated with variety of industrial effluents.
机译:砷(As)是一种众所周知的有毒准金属,已进入世界许多地方的地下水,导致严重的健康问题。抗药细菌是解决此类问题的环保工具。本研究从巴基斯坦卡苏尔工业区分离出砷(V)耐药菌。16S rDNA测序显示分离株S1和S5分别为枯草芽孢杆菌和蜡样芽孢杆菌。枯草芽孢杆菌分离株S1、S2和S8能够抵抗1.0mM Zn、Co、Ni、Pb、Cu和0.1mM Cr和Hg,细菌分离株S2、蜡样芽孢杆菌S5、S7和S8能够抵抗高达100mM的As(V)。蜡样芽孢杆菌 S5 显示最大的 As(V) 还原量高达 837.6^M (83.7%)。枯草芽孢杆菌S1的As(III)氧化最大,高达122.44^M(24.4%)。细菌分离株也被发现产生生物表面活性剂,细菌分离株枯草芽孢杆菌S1显示出最大的乳化指数(高达37.5)。这些细菌是被各种工业废水污染的场地进行生物修复的潜在候选者。

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