Identification of Syrian hamster chromosomes by acetic-saline-Giemsa (ASG) and trypsin techniques

摘要

The Syrian hamster karyotype was established by use of known banding techniques with ASG and trypsin. Each pair of chromosomes was definitely identified on the basis of banding patterns. Differences in patterns between the two techniques were limited to resolution of fine bands in some chromosomes. Examination of 4-nitroquinoline-n-oxide transformed cells after use of the trypsin technique made possible accurate identification of numerical changes in chromosome groups and of chromosomes with uncommon banding patterns which occurred subsequent to neoplastic transformation.