BACKGROUND: We have developed a technology to electrically polarize living bone. OBJECTIVE: The effects of stored electrical charge in electrical polarized bone on the facilitation of new bone formation were assayed. METHODS: Stimulated depolarized current measurement was performed in electrically polarized and nonpolarized femora of SD rats. These bone specimens were implanted into bone defects of the rat femora and fixed with a custom-made external fixator. X-ray imaging of the implant was performed every week. After 3 weeks, micro-CT scanning was performed to evaluate the displacement rate. Histological observation was performed, and the occupancy ratio of the newly formed bone was calculated from tissue specimens stained with Villanueva's Goldner method. RESULTS: There was a tendency for the displacement rate of the mnplant to be smaller and the occupancy ratio of the newly formed bone to be larger, especially at the distal end, in the polarized group compared with the nonpolarized group. The time of callus appearance was significantly earlier in the polarized group than in the nonpolarized group, and bridging callus grew from the distal to the proximal end. CONCLUSIONS: Bone specimens can be electrically polarized, and the stored electrical charge can work effectively to facilitate new bone formation.
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机译:背景:我们开发了一种对活骨进行电极化的技术。目的:分析电极化骨中储存的电荷对促进新骨形成的影响。方法:对SD大鼠的电极化和非极化股骨进行受激去极化电流测量。这些骨标本被植入大鼠股骨的骨缺损中,并用定制的外固定器固定。每周对植入物进行 X 射线成像。3周后,进行显微CT扫描以评估置换率。进行组织学观察,并从用Villanueva的Goldner方法染色的组织标本中计算新形成的骨的占用率。结果:与非极化组相比,极化组的铠骨位移率更小,新形成的骨的占用率更大,特别是在远端。极化组愈伤组织出现时间明显早于非极化组,桥接愈伤组织从远端向近端生长。结论:骨标本可电极化,储存的电荷可有效促进新骨形成。
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