The effect of different temperatures on fatty-acid synthesis and polyunsaturation in cell suspension cultures

摘要

Cell suspension cultures ofCatharanthus roseusG. Don,Glycine max(L.) Merr. andNicotiana tabacumL. were incubated with 14Cacetate, 14Coleic acid and 14Clinoleic acid at five different temperatures ranging from 15 to 35° C. When the incubation temperature was increased, 14Cacetate was incorporated preferentially into 14Cpalmitate, with a concomitant drop in 14Coleate formation. Between 15 and 20° C, 14Coleic acid accumulated inC. roseuscells. In all cultures, optimum desaturation of 14Coleic acid to 14Clinoleic acid occurred between 20 and 25° C, and inG. maxthis was also the optimal range for desaturation of 14Clinoleic acid to 14Clinolenic acid. Elongation of 14Cpalmitic acid was inhibited when cultures grown at 15° C for 25 h were subsequently incubated with 14Cacetate at 25° C. 14Coleic acid accumulated inG. maxandC. roseuscultures grown at 35° C for 25 h and subsequently incubated at 25° C. Desaturation of 14Coleic acid increased up to 25° C, but then decreased or leveled off depending on the cell line and on the temperature prior to inc