The three major fractions (II, III and V) of the five fractions of gibberellin (GA)-like activity obtained by the thin layer chromatographic purification of an extract of hazel embryos have been examined by gas liquid chromatography and combined gas chromatography-mass spectrometry. By these techniques GA1was identified as the methyl ester trimethylsilyl ether in fraction II and GA9as the methyl ester in fraction V. Both substances were clearly separated from other components by gas liquid chromatography so that accurate determination of their concentrations was possible. GA1accounted for most of the activity found by bioassay in fraction II while GA9accounted for only part of the activity of fraction V. The GA-like substances in the less biologically active thin layer chromatography fractions have not yet been identified.
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