A relative study of frequency and spectrum of chlorophyll mutations induced by physical (gamma rays) and chemical (EMS) mutagens in M_2 population was conducted in two desi (Pb2000, C44), one each of kabuli (Pbl) and desi x kabuli introgression line (CH 40/91) of chickpea (Cicer arietinum L.). The treatments included two doses each of gamma irradiation and concentrations of EMS. The overall frequencies and spectrum of four types of induced chlorophyll mutants were viridis (9.01), followed by xantha(8.61), chlorina (5.82), albina (0.5) and others (0.26). EMS treatments were found to be more efficient and effective than gamma rays in all the varieties. It appeared that recombinant of desi x kabuli chickpea introgression genotype was more responsive to chemical and physical mutagens as compared to pure desi and kabuli chickpea genotypes. The study of induced genetic variability for frequency and spectrum of chlorophyll mutations on recombinant of desi x kabuli chickpea introgression genotype isthe first report in chickpea.
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