Abstract: Two B‐cell lines, designated as HF‐1 and HF‐4, were characterised. The cell lines have complicated karyotype abnormalities including a 14;18 translocation and an 8q24 breakpoint originating from t(2;8)(p11;q24) (HF‐1) or t(1;8)(p21:q24) (HF‐4). The lines have BCL2 rearrangement and they are positive for CD19, CD20, CD22, CD39. HF‐1 is also positive for IgG, and HF‐4 is positive for IgM and IgD. On Northern blot analyses, the 2.6‐kb and 4.2‐kb transcripts corresponding to the major transcripts of CMYC and BCL2, respectively, were seen. In Western blot as well as in FACS (fluorescence‐activated cell sorting) analysis the presence of BCL2 protein in the both HF‐1 and HF‐4 cells was demonstrated. The cell lines are expected to serve as an important tool in the study of the chromosomal mechanism activating cellular oncogenes, the somatic hypermutation mechanism of antigen‐activated B cells a
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