In vitroeffect of histamine and histamine H1and H2receptor antagonists on cellular proliferation of human malignant melanoma cell lines

摘要

Histamine is an established growth factor for gastric and colorectal cancer. Contradictory data for response of melanoma to histamine have been reported. Our aims were to determine the effect of histamine and H1and H2receptor antagonists on cell growth and cyclic AMP production. Four human melanoma cell lines were cultured with a range of concentrations of histamine, and with the H2receptor antagonists cimetidine, ranitidine or famotidine, or the H1receptor antagonist diphenhydramine. Cellular proliferation was measured by the uptake of 3H-thymidine. Cyclic AMP production was also measured to determine the receptor status of the cell lines. Histamine significantly stimulated growth in two of four cell lines, with maximal stimulation at 1 x 10-8M. This effect was Inhibited by all four antagonists in a dose-dependent manner. Histamine 10-7to 10-4M also Induced a dose-dependent Increase In cyclic AMP production In the two histamine-responsive cell lines, suggesting that these cell lines express H2receptors. We conclude that there may be a role for histamine receptor antagonists in melanoma treatment and that further Investigation Is warranted.