A tissue culture was obtained from the cells around the vein of a piece of normal safflower leaf. The tissue has now been transferred monthly for two and one-half years. Growth was measured on White's, Knop's, and Heller's media with and without 0.1ensp;mgm. indole-3-acetic acid per liter and/or 10 coconut milk. Indole-3-acetic acid had no effect but coconut milk greatly enhanced growth. Heller's medium plus coconut milk was the best for growth of the leaf tissue. The addition of coconut milk to White's medium caused a decrease in percentage of dry weight of the leaf tissue culture, whereas its addition to Heller's medium had no such effect.
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