AbstractThe preparation of porous substances immobilizing enzymes with polymer matrix having various properties have been studied by radiation polymerization method. The enzyme (cellulase) was immobilized on the surface parts of porous substances such as activated carbon, molecular sieve, silica gel, and coating with hydrophilic and hydrophobic monomers. As substrate of enzyme reaction, lignocellulosic wastes such as chaff pretreated by radiation irradiation (100 Mrad) were used. The enzyme activity of the immobilized enzyme substances was markedly affected by hydrophilicity of monomer and the copolymerization of methoxypoly(ethyle glycol) methacrylate (75) and poly(ethylene glycol) dimethacrylate (25) monomer gave the most highest enzyme activity. The immobilized enzyme substances was able to hydrolyze chaff pretreated by radiation irradiation.
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