Utilization of the MHC Class I (H‐2Kb) Purified Molecule and its Synthetic Peptides for Inhibition of Kb‐Specific Suppressor T Cells and their InductionIn Vivoby the MHC Peptides

摘要

Six synthetic peptides of the MHC class I molecule corresponding to individual H‐2Kbparticipants in amino acid sequences of domains α1(peptide 1 and 2) and α2(peptides 3,4, 5,6) were selected. Kb‐specific suppressor T cells (Ts) were inducedin vivoin mice, then pretreated with a set of peptides and assayed by proliferation decrease in a three‐cell lymphocyte culture (MLC). The effector function of Ts was abolished by the complex of the α2‐domain peptides (but nol by the α1‐domain peptides) and decreased by particular peptides separately (4, 5, 6) of the α2‐domain. Both α1‐ and α2‐domain peptides. added in high concentration, decreased otherwise efficient enrichment of Ts during the absorption‐elution procedure on the syngeneie macrophage (Mφ) monolayers. A similar significant effect was observed using the purified Kbmolecule (100μg/ml) in the allogeneic Mφ monolayer. Interaction between Ts receptors and some MHC peptides indicates in effector Ts activationin vivoby induction with peptides 5 and 6 of the α2‐domain. The fine mechanisms of interaction between MHC class I molecule epitopes and T‐cell receptors of each of the T‐cell subsets sepa