Bacteria capable of utilizing ethane as their sole source of carbon and energy at 42° were readily isolated from soil samples obtained from Brunei with batch culture enrichment techniques. No ethane utilizers capable of growth at 42° were isolated, however, from soil samples obtained from Britain. An ethane‐utilizing mixed culture was grown under ammonia limitation in chemostat culture. The lipid content of the culture was inversely proportional to the dilution rate. Similarly, the amount of organic carbon excreted into the culture supernatant was inversely proportional to the growth rate and could be accounted for mainly as carbohydrate. The specific rates of ethane and oxygen consumption, carbon dioxide production and yield from ethane were directly proportional to the dilution r
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