High frequency regeneration from scutellum derived calli of Basmati rice cv. Basmati 385 and Super Basmati.

摘要

An experiment was conducted to obtain a high frequency regeneration from rice cultivars Basmati 385 and Super Basmati, which is a prerequisite for transformation protocol. Callus induction was obtained from N6 media supplemented with 2 mg 2,4-D/litre. Super Basmati exhibited high callus induction efficiency (93.3) followed by Basmati 385 (90.2). Twenty-one-day-old maintained calluses when transferred on MS medium with different combinations of auxin-cytokinin for regeneration showed 90 frequency of plant regeneration for Super Basmati with 1 mg NAA/litre and 2.5 mg benzyladenine/litre, and 83 for Basmati 385 on MS medium supplemented with 1 mg NAA/litre and 5 mg benzyladenine/litre..