Solid-state fermentation has the potential to produce inexpensive enzymes for use inhigh-volume industrial applications. Process parameters such as substrate moisture content andlength of fermentation can have a significant effect on the amount and timing of enzyme production.This study was conducted in two stages, a screening stage and an optimization stage, to determinethe effects of moisture content of the substrate, surfactant addition upon inoculation, depth of thesubstrate, and duration of fermentation on xylanase activity produced by Trichodermalongibrachiatum. Screening fermentations were conducted at 25℃, 50 and 75 wet basis moisturecontent (w.b.), 0.0 and 0.2 v/v suifactant addition, 0.5 and 1.5 cm depth of wheat bran, and 5 and10 days offermentation. Optimization fermentations were conducted at 25℃, 45, 55, and 65moisture content (w.b.), 1.0, 1.5, and 2.0 cm depth of wheat bran, and three and five days offermentation. Experiments were conducted as flill factorial experiments with three replications ofeach treatment. The optimal values of the process variables were selected based on the units ofxylanase activity produced per gram of wheat bran (U/g). Moisture content, depth of substrate, andduration of fermentation had significant main effects on the production of enzyme activity. Surfactantaddition upon inoculation had interaction effects with moisture content, and the duration offermentation by moisture content interaction also was significant. The treatment of 55 moisturecontent, 1.5 cm depth of substrate, and five days of fermentation resulted in the highest averagexylanase activity (716 U/g wheat bran).
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