Revival ofBacillus subtilisspores from biocide‐induced injury in the germination process

摘要

Spores ofBacillus subtilisNCTC 8236 were treated with glutaraldehyde, Lugol's iodine, polyvinylpyrrolidone‐iodine (PVP‐I), sodium hypochlorite or sodium dichloroisocyanurate (NaDCC). After exposure survivors were enumerated on nutrient agar containing potential revival agents (subtilisin, lysozyme, calcium dipicolinate, calcium lactate). Of these, only calcium lactate had any significant enhancing effect and then only with iodine‐treated spores. Calcium lactate (9 mmol 1−1) in nutrient broth enhanced the rate and extent of germination of iodine‐treated spores but not of spores previously subjected to glutaraldehyde, hypochlorite