Luminescence video analyzer of biological microchips

摘要

This paper describes the operating principle of a luminescence video analyzer of biochips, based on a specially developed superbroad-band projection mirror-lens-prism objective. The video analyzer makes it possible to work with diagnostic tuberculosis chips. The field of view of the video analyzer is 6 X 8 mm, and the resolution over the field is better than 80 line/mm. The sensitivity is 10 amol of dye Cy5 per cell. The layout of the analyzer and a typical scanogram of the luminescence-signal distribution over the field of view are presented. (C) 2006 Optical Society of America.