Major polypeptides associated with differentiation in psychrophilic fungi

摘要

Major polypeptides were observed upon one-dimensional sodium dodecyl sulfate gel electrophoresis of sclerotial extracts of the following psychrophiles:Myriosclerotinia borealis,Coprinus psychromorbidus,Typhula idahoensis, andTyphula incarnata. In general, the number, molecular mass, and relative proportion of these major sclerotial polypeptides varied considerably from species to species. Furthermore, in the case ofM.borealis, the major sclerotial polypeptide did not appear to be an artifact of culturing conditions since a major polypeptide of similar molecular mass was also present in sclerotia ofM.borealiscollected from the field. Generally, the major sclerotial polypeptides were visible in the sclerotial initials but were not apparent in the vegetative hyphae. Thus, these major sclerotial polypeptides appear to be expressed as a function of sclerotial development. Electrophoresis of protein extracts ofT.idahoensisandT.incarnatainitially solubilized either in sodium dodecyl sulfate or urea sample buffer indicated that the type of denaturant initially used had a profound influence on the relative proportions of the major polypeptides and the overall polypeptide profile. Isoelectric focusing of sclerotial extracts indicated that the isoelectric points of the major sclerotial polypeptides ofM.borealisranged from 6.2 to 6.7, whereas the values of the major sclerotial polypeptides of the other three species were basic and ranged from 7.0 to 7.7.