Subunit structure of the 12S protein from seeds ofBrassica juncea

摘要

The 12S globulin fromBrassica junceaseeds has been dissociated by 6ensp;Murea: 0.1ensp;Mmercaptoethanol and the resultant products have been partially separated by preparative isoelectric focusing. Species that have the isoelectric points 4.75, 5.15, 5.40, 6.25, 6.70, 7.00, 7.20, 7.70, 8.30, 8.90, and 9.15 were detected. The results of poly aery lamide-gel electrophoretic analysis and the amino acid composition of the fractions suggested that the isoelectric species were distinct subunits.