Fifty exocrine pancreatic adenocarcinomas and 57 benign tumors induced in Syrian hamsters byN-nitrosobis (2-oxopropyl)amine (BOP) were examined for the presence of argyrophil cells antiinsulin, -glucagon,-somatostatin, -pancreatic polypeptide (PP), -gastrin/CCK, -vasoactive intestinal polypeptide (VIP), and - neuron-specific enolase (NSE) reactive cells. Argyrophil— and antihormone-reactive cells were found in the normal pancreatic ducts and in the acini, as well as in hyperplastic and atypical ducts/ductules, tubular complexes, benign lesions, and in 80 of ductal adenocarcinomas. Insulin and antiNSE-reactive cells were the most common, followed in decreasing frequency by glucagon, somatostatin, and PP cells. Antigastrin-/CCK- and-VIP-reactive cells were found in two cases. Argyrophil cells were present in about 60 of the tumors with Grimelius staining and in 55 of those with Churukian-Schenk staining. Insulin cells were seen in ductal cancer that had grown into a lymph node and in the lymph node metastases of another cancer. A novel finding was the presence of argyrophil and insulin cells within the lumen of some malignant glandular structures. Coexistence of several peptide cells was found in 52 of the cancers. The presence of argyrophil and hormoneproducing cells in induced pancreatic ductal/ductular lesions further strengthens the existence of a close developmental relationship between exocrine and endocrine cells of the pancreas. Key Words: Pancreatic cancer; experimental; Syrian hamster; endocrine cells; argyrophil cells; immunohistochemistry; pancreatic peptides; BO
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