Sex differences in androgen-regulated cytochrome P450 aromatase mRNA in the rat brain

摘要

The conversion of testosterone to estradiol by cytochrome P450 aromatase (P450AROM) in the medial preoptic area is required for full expression of male sexual behavior in rats. Preoptic P450AROMactivity is stimulated by androgens through an androgen-receptor mediated mechanism that regulates P450AROMgene expression. The mechanism of enzyme induction appears to be sexually dimorphic in several species leading to greater testosterone-stimulated P450AROMactivity in males than in females. The present study was designed to determine whether the sex difference in androgen-regulated P450AROMactivity is manifested at the levels of mRNA expression. We compared the concentrations of P450AROMmRNA and enzyme activity between five different treatment groups: intact males, castrated males (CX), ovariectomized females (OVX), CX males treated with dihydrotestosterone (CX+DHT), and OVX females treated with DHT (OVX+DHT). We found that unstimulated levels of P450AROMmRNA and enzyme activity in both the preoptic area and medial basal hypothalamus were similar in the CX and OVX groups. However, when treated with equivalent doses of DHT, the levels of P450AROMmRNA and enzyme activity in both brain regions were significantly higher in males than in females (i.e., CX+DHT group>OVX+DHT group). These results demonstrate that sex differences in the regulation of P450AROMin brain are exerted pretranslationally by androgen and suggest that gender differences in androgen responsiveness play an important role in regulating gene expression in the adult rat brain.