The role temperature plays during ethylene pretreatment on subsequent induction of ethylene biosynthesis by Cellulysin or by a partially purified ethylene inducing factor (EIF) from Cellulysin in tobacco (Nicotiana tabacumL., cv. Xanthi) leaf discs was studied. Leaves were pretreated with ethylene at three temperatures (7°C, 25°C, and 40°C) followed by the induction of ethylene biosynthesis at 23°C. At 25°C, ethylene pretreatment stimulated subsequent Cellulysin-, EIF- or ACC-induced ethylene biosynthesis and conjugation of ACC. At 7°C ethylene pretreatment had little effect on subsequent Cellulysin-, EIF- or ACC-induced ethylene biosynthesis, while 40°C pretreatment inhibited subsequent Cellulysin-, EIF- or ACC-induced ethylene biosynthesis at 23°C. The high temperature (40°C) pretreatment inhibited subsequent conversion of ACC to ethylene in both air and ethylene treated
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