Identification of autoanti‐Thy‐1 antibody in the sera of BALB/c mice immune to P 1798 lymphoma

摘要

AbstractThe specificity was established for the anti‐thymocyte autoantibody which appeared in BALB/c mice (BAA) during immunization to the P 1798 lymphoma. The results indicate that BAA is specific for the Thy‐1 alloantigen of murine T cells. By immunoprecipitation of125I‐labeled A/J thymocyte surface antigens from freeze‐thaw lysates and electrophoretic resolution of the immunoprecipitates on sodium dodecyl sulfate polyacrylamide gels, the BAA and congenic anti‐Thy‐1.1 or 1.2 were shown to be specific for determinants on the same antigens. Specificity of BAA for Thy‐1.2 was shown also by quantitative absorption studies. Coating P 1798 lymphoma cells with either BAA or anti‐Thy‐1.2 reduced the absorptive capacity of these cells for the same or the reciprocal antiserum. Coating P 1798 with an antiserum to P 1798 which was indifferent to the Thy‐1.2 alloantigen did not diminish the absorptive capacity of these cells for either BAA or anti‐Thy‐1.2. These studies have provided firm evidence that BAA is specific for determinants o