Lipids in the Classification and Identification of Coryneform Bacteria Containing Peptidoglycans Based on 2, 4‐diaminobutyric Acid

摘要

Strains of 2, 4‐diaminobutyric acid‐containing coryneform bacteria were degraded by acid methanolysis and the non‐hydroxylated fatty acid esters released examined by thin‐layer and gas chromatography. The major fatty acid structural types were straight‐chain,anteiso‐ andiso‐methyl branched‐chain acids. Polar lipids of the test strains were examined by two‐dimensional thin‐layer chromatography. All strains possessed very characteristic polar lipid patterns consisting of diphosphatidylglycerol, phosphatidylglycerol and a number of uncharacterized glycolipids. Menaquinones (vitamin K) were the sole isoprenoid quinones detected in the test strains.Corynebacterium insidiosum, Cor. michiganense, Cor. nebraskenseandCor. sepedonicumcontained unsaturated menaquinones with nine isoprene units, whereas unsaturated menaquinones with 10 isoprene units predominated in strains ofCor. iranicumandCor. triticiand a strain labelledArthrobactersp. The single strain ofCor. aquaticumexamined contained comparable amounts of menaquinones with 10 and 11 isoprene units whereas strains ofCor. mediolanumandFlavobacterium dehydrogenanscontained major amounts of menaquinones with 11 and 12 isoprene units. The results of the present study indicate that lipid markers may be of considerable value in the classification and identification of 2, 4‐diaminobutyric acid‐containing phytopathogenic and saprophy