In vitro germination and pollen tube growth of maize (Zea mays) pollen. IV. Effects of the fertility restoringRf1locus

摘要

Pollen grains from the near-isogenic inbreds, Oh 43Rf1Rf1, Oh 43rf1rf1, 106Rf1Rf1, and 106rf1rf1were cultured on an artificial medium containing 15 sucrose and 0.6 bacto-agar supplemented with all possible combinations of two levels (0.00 and 0.03) of calcium nitrate (Ca) and two levels (0.00 and 0.01) of boric acid (B). At certain combinations of Ca and B, significant differences between theRf1Rf1andrf1rf1genotypes were obtained for germination percentage, rupturing percentage, and pollen tube length. However, the effect of theRf1andrf1alleles was not consistent in magnitude for both inbreds. Apparently, theRf1andrf1alleles can alter certain in vitro germination characteristics but genetic background is also involved.