The in situ occurrence and localization of 3-hydroxy oxylipins (fatty acids) in selected members of the Lipomycetaceae were mapped with immunofluorescence microscopy. As was found previously in Dipodascopsis uninucleata, fluorescence was associated with the meiospores of all the strains tested. These results further highlight this yeast family as a rich source of 3-hydroxy oxylipins, which have potent biological activity in mammalian cells and are hence of biological interest. In addition, immunogold labelling of Dipodascopsis uninucleata revealed lipid globules with a high affinity for the antibody specific to 3-hydroxy fatty acids. These lipids were produced during the generative stage. These observations may aid in the further optimization and extraction of these novel compounds.
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